General Information of Synthetic Binding Protein (SBP) (ID: SBP001144)
SBP Name
Nanobody anti-VGLUT1 clone 1
Synonyms
Nb1
Molecular Weight 12.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli MC1061
Selection Method Phage display
Highest Status Research
Sequence Length 120
SBP Sequence
>Nanobody anti-VGLUT1 clone 1
QVQLQESGGGLVQAGGSLRLSCVGSGSMSSINGMGWYRQAPGREREFLAAISWSGGSTYY
AGSVKGRSTISRDLAKNTGYLEMTRVKPEDTAVYYCAASETGLPYEYDYWGQGTQVTVSS
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Vesicular glutamate transporter 1
BTS Info
Inhibitor Biochemical agents N.A. University of Zurich [1]
References
1 Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport. Biochemistry. 2017 Aug 1;56(30):3962-3971.