General Information of Synthetic Binding Protein (SBP) (ID: SBP001094)
SBP Name
Nanobody anti-MBP clone 3
Synonyms
Sb_MBP#3; L2_D09 (a-MBP#3)
Molecular Weight 13.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
PDB ID 5M15
Sequence Length 128
SBP Sequence
>Nanobody anti-MBP clone 3
GPSQVQLVESGGGSVQAGGSLRLSCAASGKIHEIGYLGWFRQAPGKEREGVAALMTFQGQ
TYYADSVKGRFTVSLDNAKNTVYLQMNSLKPEDTALYYCAAAYWGKQSPLISWDYSYWGQ
GTQVTVSA
3D Structure
Experimentally Validated Structure
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Template Name Convex scaffold
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Maltose/maltodextrin-binding periplasmic protein
BTS Info
Binder Tools for the conformational trapping of membrane proteins Kd: 502 nM University of Zurich [1]
References
1 Synthetic single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7:e34317.