General Information of Synthetic Binding Protein (SBP) (ID: SBP001073)
SBP Name
Nanobody anti-Lactose permease clone 9047
Synonyms
Nb9047
Molecular Weight 13.5 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
PDB ID 6C9W
Sequence Length 124
SBP Sequence
>Nanobody anti-Lactose permease clone 9047
VQLVESGGGLVQAGDSLRLSCAASGGTFSTFNMGWFRQDLGKEREFVAAIRWTGGRAYYG
DSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCARQGTNGGGYSEATSYNYWGQGTQV
TVSS
3D Structure
Experimentally Validated Structure
Click to Save PDB File
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Lactose permease
BTS Info
Binder Research tool Kd: 5300 nM University of California [1]
References
1 Crystal Structure of a ligand-bound LacY-Nanobody Complex. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8769-8774.