General Information of Synthetic Binding Protein (SBP) (ID: SBP001072)
SBP Name
Nanobody anti-Lactose permease clone 9039
Synonyms
Nb9039
Molecular Weight 14.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
PDB ID 5GXB
Sequence Length 130
SBP Sequence
>Nanobody anti-Lactose permease clone 9039
MQVQLVESGGRLVQAGDSLRLSCAASGRTFTTYLMGWFRQAPGKEREFVAAIRWSGGSTY
YADSVKGRFTISRDNAKNTVYLQMNSLKLEDTAVYYCAAAARPSYSGDYGYTEALRYDYW
GQGTQVTVSS
3D Structure
Experimentally Validated Structure
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Template Name Chain B of PDBID 4KRP
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Lactose permease
BTS Info
Binder Tools for stabilizing this state of the protein Kd: 15000 nM Tsinghua University [1]
References
1 Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425.