General Information of Synthetic Binding Protein (SBP) (ID: SBP001062)
SBP Name
Nanobody anti-BtuF clone 9
Synonyms
Nb9
Molecular Weight 15.7 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli WK6
Selection Method Phage display
Highest Status Research
PDB ID 5OVW
Sequence Length 149
SBP Sequence
>Nanobody anti-BtuF clone 9
MKYLLPTAAAGLLLLAAQPAMAQMQLVESGGGLVQPGGSLRLSCAAPESTLDDYAIGWFR
QAPGKEREGVSCIGSSGDSTNYADSVKGRFTVSRDNAKNTVYLQMNDLRPEDTAVYYCAA
AHRIFGGCLVIHSSGYVSWGQGTPVTVSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Vitamin B12-binding protein
BTS Info
Inhibitor Research tool Kd: 0.94 nM ETH Zurich [1]
References
1 Structural basis of nanobody-mediated blocking of BtuF, the cognate substrate-binding protein of the Escherichia coli vitamin B12 transporter BtuCD. Sci Rep. 2017 Oct 30;7(1):14296.