General Information of Synthetic Binding Protein (SBP) (ID: SBP001051)
SBP Name
Nanobody anti-GFP enhancer
Synonyms
GFP-specific nanobody Enhancer
Molecular Weight 13.7 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Highest Status Research
PDB ID 3K1K
Sequence Length 123
SBP Sequence
>Nanobody anti-GFP enhancer
MAQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRS
SYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSSHHH
HHH
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Modulator Tools for manipulate and study protein conformations Kd: 0.59 nM Ludwig-Maximilians University Munich [1]
References
1 Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol. 2010 Jan;17(1):133-8.