Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000805)
SBP Name	WW domain anti-peptide mutant W17F
Synonyms	WW domain mutant W17F
Molecular Weight	7.1 kDa
Thermal Denaturation TEMP	50.3 ℃
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli BL21 (DE3)
Highest Status	Research
Sequence Length	57
SBP Sequence	>WW domain anti-peptide mutant W17F GSQSSFEIPDDVPLPAGFEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSRI HRD
3D Structure
	Computationally Modelled Structure
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Template Name	hYAP

Protein Scaffold Information of This SBP
Scaffold ID	PS067 Scaffold Info					[1]
Scaffold Name	WW domain
Scaffold Class	Non-Antibody
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
PY-ligand	BTS Info	Binder	Research tool	Kd: 15100 nM	The Skaggs Institute of Chemical Biology	[1]

References

1

Characterization of the structure and function of W --> F WW domain variants: identification of a natively unfolded protein that folds upon ligand binding. Biochemistry. 1999 Oct 26;38(43):14338-51.