Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000558)
SBP Name	Knottin anti-Integrin 2bO
Synonyms	Knottin 2bO
Molecular Weight	4.4 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Pichia pastoris GS115
Selection Method	Yeast display
Highest Status	Research
Sequence Length	38
SBP Sequence	>Knottin anti-Integrin 2bO GCVRLHESCLGQQVPCCDPAATCYCKKRGDWKERCYCR
Sequence Description	Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VIII form disulfide bonds; Cys VI and Cys VII form disulfide bonds.
3D Structure
	Computationally Modelled Structure
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Template Name	AgRP
Template Sequence Description	Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VIII form disulfide bonds; Cys VI and Cys VII form disulfide bonds.

Protein Scaffold Information of This SBP
Scaffold ID	PS042 Scaffold Info					[1]
Scaffold Name	Knottin
Scaffold Class	Non-Antibody
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Integrin alpha-IIb-beta-3	BTS Info	Inhibitor	Tools as inhibitors of platelet aggregation	Kd: 90 nM	Stanford University	[1]

References

1

Cystine-knot peptides engineered with specificities for (IIb)(3) or (IIb)(3) and (v)(3) integrins are potent inhibitors of platelet aggregation. J Mol Recognit. Jan-Feb 2011;24(1):127-35.