Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000526)
SBP Name
Knottin anti-CTLA-4 MC-CT-020
Synonyms
Knottin MC-CT-020
Molecular Weight 4.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Yeast display
Highest Status Research
Sequence Length 40
SBP Sequence
>Knottin anti-CTLA-4 MC-CT-020
SAGGAYKCSYRDRKSKSKDRCRRDSDCPGHCICRGNGYCG
Sequence Description Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VI form disulfide bonds.
3D Structure
Computationally Modelled Structure
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Template Name oMCoTI-
Template Sequence Description Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VI form disulfide bonds.
Protein Scaffold Information of This SBP
Scaffold ID PS042
Scaffold Info
[1]
Scaffold Name Knottin
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Cytotoxic T-lymphocyte protein 4
BTS Info
Binder Cancer metastasis [ICD-11: 2E2Z] Kd: 17000 nM Technical University of Darmstadt [1]
References
1 Cystine-knot peptides targeting cancer-relevant human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). J Pept Sci. 2015 Aug;21(8):651-60.