General Information of Synthetic Binding Protein (SBP) (ID: SBP000505)
SBP Name
Affibody anti-Monoclonal antibody Z(mab22)
Synonyms
Affibody Z(mab22)
Molecular Weight 6.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli Rosetta (DE3)
Selection Method Ribosome display
Highest Status Research
Sequence Length 58
SBP Sequence
>Affibody anti-Monoclonal antibody Z(mab22)
VDNKFNKEAWRAHMEIMCLPNLNWSQTTAFIFSLCDDPSQSANLLAEAKKLNDAQAPK
3D Structure
Computationally Modelled Structure
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Template Name Z domain of staphylococcal protein A
Protein Scaffold Information of This SBP
Scaffold ID PS004
Scaffold Info
[1]
Scaffold Name Affibody
Scaffold Class Non-Antibody
Fold Type Three Alpha-Helices
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Monoclonal antibody 1 of IgG1
BTS Info
Binder Tools for recognizing IgG1 Kd: 1300 nM Royal Institute of Technology [1]
Monoclonal antibody 2 of IgG1
BTS Info
Binder Tools for recognizing IgG1 Kd: 487 nM Royal Institute of Technology [1]
Monoclonal antibody 3 of IgG1
BTS Info
Binder Tools for recognizing IgG1 Kd: 8.1 nM Royal Institute of Technology [1]
References
1 Ribosome display selection of a murine IgG? Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies. Mol Biotechnol. 2011 Jul;48(3):263-76.