General Information of Synthetic Binding Protein (SBP) (ID: SBP000305)
SBP Name
SWEEPin anti-GFPuv clone 40
Synonyms
SWEEPin GFP-40
Molecular Weight 11.5 kDa
Thermal Denaturation TEMP 63.5? ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
PDB ID 7CD7; 7CD8
Sequence Length 99
SBP Sequence
>SWEEPin anti-GFPuv clone 40
GSGEWEIIDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPSMKKTIYYANSRGMIKGY
EYQLYVYASDKLFRADISEDYQHYPHRKLLRFNGPVPPP
3D Structure
Experimentally Validated Structure
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Template Name scMonellin
Protein Scaffold Information of This SBP
Scaffold ID PS059
Scaffold Info
[1]
Scaffold Name SWEEPin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Research tool Kd: 24000 nM Okayama University [1]
References
1 A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins. J Biochem. 2021 Jan 2;mvaa147.