General Information of Synthetic Binding Protein (SBP) (ID: SBP000055)
SBP Name
Monobody anti-MBP YSX1
Synonyms
Monobody YSX1
Molecular Weight 10.2 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Yeast display; Surface plasmon resonance
Highest Status Research
PDB ID 3CSB; 6APX
Sequence Length 94
SBP Sequence
>Monobody anti-MBP YSX1
GSSVPTNLEVVAATPTSLLISWDAYSSSYYVYYYRITYGETGGNSPVQEFTVPGSKSTAT
ISGLKPGVDYTITVYAGHYLYGLLSSPISINYRT
3D Structure
Experimentally Validated Structure
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Template Name Fibronectin type III domain (FN3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Maltose/maltodextrin-binding periplasmic protein
BTS Info
Binder Research tool Kd: 5.7 nM University of Chicago; National Cancer Institute at Frederick [1]
References
1 A dominant conformational role for amino acid diversity in minimalist protein-protein interfaces. J Mol Biol. 2008 Aug 29;381(2):407-18.