Details of the SBP
| General Information of Synthetic Binding Protein (SBP) (ID: SBP000044) | ||||||
|---|---|---|---|---|---|---|
| SBP Name |
Monobody anti-GFP GL4
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| Synonyms |
Monobody GL4
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| Molecular Weight | 10.4 kDa | |||||
| Design Method | Traditional methods (Site-directed mutagenesis and/or Directed evolution) | |||||
| Expression System | Yeast | |||||
| Selection Method | Phage display; Yeast display | |||||
| Highest Status | Research | |||||
| Sequence Length | 94 | |||||
| SBP Sequence |
>Monobody anti-GFP GL4
VSSVPTKLEVVAATPTSLLISWDASSSSVSYYRITYGETGGNSPVQEFTVPGSSSTATIS GLSPGVDYTITVYAYWWYRYYYYSYSPISINYRT |
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| 3D Structure | ||||||
| Computationally Modelled Structure | ||||||
| Click to Save PDB File | ||||||
| Template Name | Fibronectin type III domain (FN3) | |||||
| Template Sequence Description | X denotes a mixture of 30% Tyr, 15% Ser, 10% Gly, 5% Phe, 5% Trp and 2.5% each of all the other amino acids except for Cys; B denotes a mixture of Gly, Ser and Tyr; J denotes a mixture of Ser and Tyr. | |||||
| Protein Scaffold Information of This SBP | ||||||
|---|---|---|---|---|---|---|
| Scaffold ID | PS047 | [1] | ||||
| Scaffold Name | Monobody | |||||
| Scaffold Class | Non-Antibody | |||||
| Fold Type | Beta-Sheets + Loops | |||||
| Binding Target(s) of This SBP (BTS) |
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| BTS Name | Details | Mechanism | Application | Affinity | Research Organization | Ref |
|---|---|---|---|---|---|---|
| Green fluorescent protein | Binder | Tools for identifying the unanticipated mode of monobody-target interactions | Kd: 478 nM | University of Chicago | [1] | |