Details of the SBP
| General Information of Synthetic Binding Protein (SBP) (ID: SBP000011) | ||||||
|---|---|---|---|---|---|---|
| SBP Name |
Monobody anti-KRas/HRas NS1
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| Synonyms |
Monobody NS1
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| Molecular Weight | 10.4 kDa | |||||
| Design Method | Traditional methods (Site-directed mutagenesis and/or Directed evolution) | |||||
| Expression System | Escherichia coli BL21 (DE3) | |||||
| Selection Method | Phage display and yeast display | |||||
| Highest Status | Research | |||||
| PDB ID | `5E95 | |||||
| Sequence Length | 97 | |||||
| SBP Sequence |
>Monobody anti-KRas/HRas NS1
GSVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGGNSPVQKFEVPGSKSTAT ISGLKPGVDYTITVYAWGWHGQVYYYMGSPISINYRT |
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| 3D Structure | ||||||
| Experimentally Validated Structure | ||||||
| Click to Save PDB File | ||||||
| Protein Scaffold Information of This SBP | ||||||
|---|---|---|---|---|---|---|
| Scaffold ID | PS047 | [1] , [2] | ||||
| Scaffold Name | Monobody | |||||
| Scaffold Class | Non-Antibody | |||||
| Fold Type | Beta-Sheets + Loops | |||||
| Binding Target(s) of This SBP (BTS) |
|---|
| BTS Name | Details | Mechanism | Application | Affinity | Research Organization | Ref |
|---|---|---|---|---|---|---|
| GTPase KRas | Inhibitor | Pancreas cancer [ICD-11: 2C10.Z] | Kd: 65 nM | Medical University of South Carolina; University of Illinois at Chicago | [1] , [2] | |
| GTPase HRas | Inhibitor | Pancreas cancer [ICD-11: 2C10.Z] | Kd: 15 nM | Medical University of South Carolina; University of Illinois at Chicago | [1] , [2] | |