General Information of Binding Target of SBP (BTS) (ID: ST00304)
BTS Name
Nucleoprotein
Synonyms
Ebola NP; eNP; Nucleocapsid protein; Protein N
BTS Type
Protein
Family
Filoviruses nucleoprotein family
Gene Name
NP
Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Function
Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response VP35 binds to and stabilizes monomeric NP, keeping it soluble Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid Recruits also host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1
UniProt ID
P18272
UniProt Entry
NCAP_EBOZM
PFam
PF05505
Gene ID
911830
Sequence
MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVYQVNNLEEICQLIIQAF
EAGVDFQESADSFLLMLCLHHAYQGDYKLFLESGAVKYLEGHGFRFEVKKRDGVKRLEEL
LPAVSSGKNIKRTLAAMPEEETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHA
EQGLIQYPTAWQSVGHMMVIFRLMRTNFLIKFLLIHQGMHMVAGHDANDAVISNSVAQAR
FSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNEVNSFKAALSSLAKHGEYAPFARLL
NLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEAEKQLQQYAES
RELDHLGLDDQEKKILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITAASLPKTSGH
YDDDDDIPFPGPINDDDNPGHQDDDPTDSQDTTIPDVVVDPDDGSYGEYQSYSENGMNAP
DDLVLFDLDEDDEDTKPVPNRSTKGGQQKNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHA
SAPLTDNDRRNEPSGSTSPRMLTPINEEADPLDDADDETSSLPPLESDDEEQDRDGTSNR
TPTVAPPAPVYRDHSEKKELPQDEQQDQDHTQEARNQDSDNTQSEHSFEEMYRHILRSQG
PFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQF
YWPVMNHKNKFMAILQHHQ
Sequence Length
739
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
VNAR anti-NP DSTL096 Research Binder N.A. Tools for detecting Ebolavirus infection
SBP Info
[1]
VNAR anti-NP DSTL097 Research Binder N.A. Tools for detecting Ebolavirus infection
SBP Info
[1]
References
1 Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries. Mol Immunol. 2011 Sep;48(15-16):2027-37.