General Information of Binding Target of SBP (BTS) (ID: ST00242)
BTS Name
SsrA-binding protein
Synonyms
Small protein B
BTS Type
Protein
Family
SmpB family
Gene Name
smpB
Organism
Aeromonas veronii
Function
Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.
UniProt ID
A0A0T6N165
UniProt Entry
A0A0T6N165_9GAMM
PFam
PF01668
Gene ID
60844630
Sequence
MSKKNSKNKAGSSTIALNRTARHEYFIEEKIEAGLSLQGWEVKSLRAGKANISEAYVIFR
DGEAYLFGSSFLPLQAASSHVVCDPTRTRKLLLSRRELDKLESLIARQGYTIVPLALYWK
QCWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKHRG
Sequence Length
162
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
Peptide aptamer anti-SmpB clone 1 Research Inhibitor Kd: 691 nM Aeromonas veronii infection
SBP Info
[1]
References
1 Targeting Inhibition of SmpB by Peptide Aptamer Attenuates the Virulence to Protect Zebrafish against Aeromonas veronii Infection. Front Microbiol. 2017 Sep 13;8:1766.