General Information of Binding Target of SBP (BTS) (ID: ST00233)
BTS Name
Replication-associated protein
Synonyms
Rep; EC 2.7.7.-; EC 3.1.21.-; Protein AC1; Protein AL1
BTS Type
Protein
Family
Geminiviridae Rep protein family
Organism
Tomato golden mosaic virus (strain Yellow vein) (TGMV)
Function
Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
UniProt ID
P03567
UniProt Entry
REP_TGMVY
PFam
PF00799 ; PF08283
Sequence
MPSHPKRFQINAKNYFLTYPQCSLSKEESLSQLQALNTPINKKFIKICRELHEDGQPHLH
VLIQFEGKYCCQNQRFFDLVSPTRSAHFHPNIQRAKSSSDVKTYIDKDGDTLVWGEFQVD
GRSARGGCQTSNDAAAEALNASSKEEALQIIREKIPEKYLFQFHNLNSNLDRIFDKTPEP
WLPPFHVSSFTNVPDEMRQWAENYFGKSSAARPERPISIIIEGDSRTGKTMWARSLGPHN
YLSGHLDLNSRVYSNKVEYNVIDDVTPQYLKLKHWKELIGAQRDWQTNCKYGKPVQIKGG
IPSIVLCNPGEGASYKVFLDKEENTPLKNWTFHNAKFVFLNSPLYQSSTQSS
Sequence Length
352
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
Peptide aptamer anti-Rep A22 Research Binder N.A. Tomato yellow leaf curl virus infection; Tomato mottle virus infection
SBP Info
[1]
Peptide aptamer anti-Rep A64 Research Binder N.A. Tomato yellow leaf curl virus infection; Tomato mottle virus infection
SBP Info
[1]
References
1 Peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato. J Virol. 2013 Sep;87(17):9691-706.