Details of the BTS

General Information of Binding Target of SBP (BTS) (ID: ST00121)
BTS Name
Aminoglycoside 3'-phosphotransferase
Synonyms
EC 2.7.1.95; Fragment
BTS Type
Protein
Family
Aminoglycoside phosphotransferase family
Organism
Escherichia coli
UniProt ID
W5U086
UniProt Entry
W5U086_ECOLX
PFam
PF01636
Sequence
LIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLP
VPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPY
TNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLG
DSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWE
KIKYYILLDELF
Sequence Length
252
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
DARPin anti-APH(3)IIIa AR_3b Research Inhibitor Kd: 0.5 nM Tools as biosensors and selective protein therapeutics
SBP Info
 		[1], [2]
DARPin anti-APH(3)IIIa clone 3a Research Inhibitor Kd: 1.7 nM Research tool
SBP Info
 		[3]
References
1 Modular protein switches derived from antibody mimetic proteins. Protein Eng Des Sel. 2016 Feb;29(2):77-85.
2 Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem. 2005 Jul 1;280(26):24715-22.
3 Chaperone-assisted crystallography with DARPins. Structure. 2008 Oct 8;16(10):1443-53.