Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS048)
PSS Name	Nanobody
Synonyms	Variable domains of the heavy chain of the heavy-chain antibody; Single-domain antigen-binding fragments of camelid antibodies; Single-domain antibodies from the camelid family; Camelid single-domain antibody; Xaperone; Sybody; VHH
Diagrammatic Sketch
Highest Status	Marketed
Class	Antibody fragment
Fold Type	Beta-Sheets + Loops
Thermal Denaturation TEMP	46-90℃
Sequence Length	92-175
Molecular Weight	11-19 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Camelid heavy chain antibody	Camelid HcAB		4M3K	N.A.	Camelus dromedarius; Synthetic construct

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Nanobody Caplacizumab	Marketed	N.A.	N.A.	N.A.	SBP Info	[1], [2], [3]
Nanobody M6495	Phase I	N.A.	N.A.	N.A.	SBP Info	[4]
Nanobody anti-AT1R AT110i1	Research	Nb.AT110	Escherichia coli	Yeast display	SBP Info	[5]
Nanobody anti-AT1R AT110i1le	Research	Nb.AT110i1	N.A.	N.A.	SBP Info	[6]
Nanobody anti-BasC clone 74	Research	Chain A of PDBID 5H8D	Escherichia coli WK6	Phage display	SBP Info	[7]
Nanobody anti-beta2AR clone 6B9	Research	Nb80	Escherichia coli BL21 (DE3)	Yeast display	SBP Info	[8]
Nanobody anti-C4b hC4Nb8	Research	N.A.	Bacteria	Phage display	SBP Info	[9]
Nanobody anti-CD70 Nb-2B3	Research	N.A.	Escherichia coli	phage display	SBP Info	[10]
Nanobody anti-CD70 Nb-3B6	Research	N.A.	Escherichia coli	phage display	SBP Info	[10]
Nanobody anti-E2 glycoprotein Nb-2E8	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Nanobody anti-E2 glycoprotein Nb-3C5	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Nanobody anti-E2 glycoprotein UNbC5-1	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[12]
Nanobody anti-E2 glycoprotein UNbC5-2	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[12]
Nanobody anti-Fab	Research	N.A.	Escherichia coli BL21	N.A.	SBP Info	[13], [14]
Nanobody anti-GlyT1 clone 1	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 2	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 3	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 4	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 5	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 6	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GlyT1 clone 7	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-GPVI Nb2	Research	N.A.	Escherichia coli	N.A.	SBP Info	[16]
Nanobody anti-Gs Nb35	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[17], [18]
Nanobody anti-HER2 MIRC213	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[19]
Nanobody anti-HER2 MIRC213-213	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[19]
Nanobody anti-HER2 MIRC213-709	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[19]
Nanobody anti-MBP clone 1	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-MBP clone 2	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-MBP clone 3	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-SARS-CoV-2 C1	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[20]
Nanobody anti-SARS-CoV-2 C5	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[20]
Nanobody anti-SARS-CoV-2 C5G2	Research	natural nanobody (PDBID 1ZVH)	N.A.	Phage display	SBP Info	[21]
Nanobody anti-SARS-CoV-2 clone 14	Research	N.A.	Escherichia coli MC1061	N.A.	SBP Info	[22]
Nanobody anti-SARS-CoV-2 clone 15	Research	N.A.	Escherichia coli	N.A.	SBP Info	[22]
Nanobody anti-SARS-CoV-2 clone 31	Research	N.A.	Escherichia coli MC1061	Ribosome display	SBP Info	[23]
Nanobody anti-SARS-CoV-2 clone Ty1	Research	Nb14	FreeStyle 293F cells	Phage display	SBP Info	[24]
Nanobody anti-SARS-CoV-2 F2	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[20]
Nanobody anti-SARS-CoV-2 H3	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[20]
Nanobody anti-SARS-CoV-2 MR17-K99Y	Research	N.A.	N.A.	N.A.	SBP Info	[25]
Nanobody anti-SARS-CoV-2 Nb-007	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[26]
Nanobody anti-SARS-CoV-2 Nb-015	Research	N.A.	N.A.	Phage display	SBP Info	[27]
Nanobody anti-SARS-CoV-2 Nb-021	Research	N.A.	N.A.	Phage display	SBP Info	[27]
Nanobody anti-SARS-CoV-2 Nb-H6	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[28]
Nanobody anti-SARS-CoV-2 NM1226	Research	N.A.	Escherichia coli	Phage display	SBP Info	[29]
Nanobody anti-SARS-CoV-2 NM1228	Research	N.A.	Escherichia coli	Phage display	SBP Info	[29]
Nanobody anti-SARS-CoV-2 NM1230	Research	N.A.	Escherichia coli	Phage display	SBP Info	[29]
Nanobody anti-SEB Aa	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Ac	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Ad	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Ca	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Cb	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Cc	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB Cd	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB D9	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SEB E2	Research	Consensus sequence	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[30]
Nanobody anti-SLC29A1 clone 1	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 10	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 11	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 12	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 13	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 14	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 15	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 16	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 17	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 18	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 19	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 20	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 21	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 22	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 23	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 24	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 25	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 26	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 27	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 28	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 29	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 3	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 30	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 31	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 32	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 33	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 34	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 35	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 36	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 37	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 38	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 39	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 4	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 40	Research	Convex scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 5	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 6	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 7	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 8	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 clone 9	Research	Loop scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 Sb_TM1	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TM287/288 Sb_TM2	Research	Concave scaffold	Escherichia coli	Ribosome display	SBP Info	[15]
Nanobody anti-TssK nbK18	Research	N.A.	Escherichia coli	Phage display	SBP Info	[31]
Nanobody anti-TssK nbK27	Research	N.A.	Escherichia coli	Phage display	SBP Info	[31]
Nanobody anti-US28Nb7 B1	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[32]
Nanobody anti-XKR9 Sb1	Research	VHH D2-L24	Escherichia coli WK6	Phage display	SBP Info	[33]
Nanobody anti-AaHII hu2NbAahII10-FERG	Research	NbAahII10	beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII hu2NbAahII10-FERG-C/S	Research	NbAahII10	beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII hu3NbAahII10-FERG	Research	NbAahII10	beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII hu3NbAahII10FGLGC/S	Research	NbAahII10	beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-ADRB2 clone c200	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c201	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c202	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c203	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c204	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c205	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c206	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c207	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c208	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c209	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c210	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c211	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-ADRB2 clone c212	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-AT1R AT118	Research	N.A.	Escherichia coli BL21 (DE3)	Saccharomyces cerevisiae yeast display	SBP Info	[36]
Nanobody anti-AT1R AT118i4	Research	N.A.	Escherichia coli BL21 (DE3)	Saccharomyces cerevisiae yeast display	SBP Info	[36]
Nanobody anti-AT1R AT120	Research	N.A.	Escherichia coli BL21 (DE3)	Saccharomyces cerevisiae yeast display	SBP Info	[36]
Nanobody anti-BamA B12	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[37]
Nanobody anti-BamA E6	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[37]
Nanobody anti-BamA F7	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[37]
Nanobody anti-BC2T clone BC2-R106E	Research	Nb BC2	Escherichia coli	N.A.	SBP Info	[38]
Nanobody anti-BC2T clone BC2-R106S	Research	Nb BC2	Escherichia coli	N.A.	SBP Info	[38]
Nanobody anti-BCII_beta_lactamase cAbBCII10-0-0-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-0-0-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-0-1-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-0-1-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-1-0-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-1-0-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-1-1-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BCII_beta_lactamase cAbBCII10-1-1-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-BtuF clone 10	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[40]
Nanobody anti-BtuF clone 7	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[40]
Nanobody anti-BtuF clone 9	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[40]
Nanobody anti-CD72 clone B5	Research	N.A.	CAR+ T cells	Yeast display	SBP Info	[41]
Nanobody anti-CFTR D12	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[42]
Nanobody anti-CFTR G3a	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[42]
Nanobody anti-CFTR T27	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[42]
Nanobody anti-CFTR T2a	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[42]
Nanobody anti-CFTR T4	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[42]
Nanobody anti-Cry1Ac P10	Research	N.A.	Escherichia coli Rosetta	Phage display	SBP Info	[43]
Nanobody anti-Cry1Ac P24	Research	N.A.	Escherichia coli Rosetta	Phage display	SBP Info	[43]
Nanobody anti-Cry1Ac P72	Research	N.A.	Escherichia coli Rosetta	Phage display	SBP Info	[43]
Nanobody anti-CyaA-Hly clone 2	Research	N.A.	Escherichia coli	Phage display	SBP Info	[44]
Nanobody anti-CyaA-Hly clone 37	Research	N.A.	Escherichia coli	Phage display	SBP Info	[44]
Nanobody anti-CyaA-Hly VH18	Research	N.A.	Escherichia coli	Phage display	SBP Info	[44]
Nanobody anti-CyaA-Hly VH5	Research	N.A.	Escherichia coli	Phage display	SBP Info	[44]
Nanobody anti-DMT clone 6616	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[45]
Nanobody anti-DtpA N00	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[46]
Nanobody anti-DtpA N21	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[46]
Nanobody anti-DtpA N87	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[46]
Nanobody anti-DtpA N89	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[46]
Nanobody anti-DtpA N93	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[46]
Nanobody anti-ELIC clone 21	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[47]
Nanobody anti-ELIC clone 22	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[47]
Nanobody anti-ELIC clone 72	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[48]
Nanobody anti-GABA(A) beta3 clone 25	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[49]
Nanobody anti-GFP cAbGFP4-0-0-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-GFP cAbGFP4-1-0-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-GFP cAbGFP4-1-0-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-GFP cAbGFP4-1-1-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-GFP cAbGFP4-1-1-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-GFP LaG-10	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-11	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-12	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-14	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-16	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-17	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-18	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-19	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-2	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-21	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-24	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-26	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-27	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-29	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-3	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-30	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-35	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-37	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-41	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-42	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-43	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-5	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-6	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-8	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG-9	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP LaG16-G4S-2	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-GFP pcNB1	Research	NB1	Escherichia coli	N.A.	SBP Info	[51], [52]
Nanobody anti-GFP pcNB2	Research	NB2	Escherichia coli	N.A.	SBP Info	[51], [52]
Nanobody anti-GFP pcNB3	Research	NB3	Escherichia coli	N.A.	SBP Info	[51], [52]
Nanobody anti-GP EBOV-GP-A8	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-B5	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-C12	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-D1	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-E7	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-G11	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-G3	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-G6	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-GP-H7	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GP EBOV-VLP-B11	Research	N.A.	Escherichia coli	Phage display	SBP Info	[53]
Nanobody anti-GPC3 G10	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[54]
Nanobody anti-GPC3 G11	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[54]
Nanobody anti-GPC3 G64	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[54]
Nanobody anti-GPC3 G8	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[54]
Nanobody anti-GSN clone 11	Research	N.A.	Escherichia coli	Southern screening	SBP Info	[55]
Nanobody anti-HIF-1A M1	Research	VHH212	Prokaryotic cells	N.A.	SBP Info	[56]
Nanobody anti-HIF-1A M3	Research	VHH212	Prokaryotic cells	N.A.	SBP Info	[56]
Nanobody anti-HSA clone b201	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[35]
Nanobody anti-IrtAB Syb_NL5	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[57]
Nanobody anti-Lactose permease clone 9039	Research	Chain B of PDBID 4KRP	Escherichia coli	N.A.	SBP Info	[58]
Nanobody anti-mCherry LaM-1	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-mCherry LaM-2	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50], [59]
Nanobody anti-mCherry LaM-3	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-mCherry LaM-4	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-mCherry LaM-6	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-mCherry LaM-8	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
Nanobody anti-MERS-CoV clone 55	Research	N.A.	Pichia pastoris	Phage display	SBP Info	[60]
Nanobody anti-P-gp clone 592	Research	Chain A of PDBID 1HCV	Escherichia coli WK6	N.A.	SBP Info	[61]
Nanobody anti-PglK clone 87	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[62]
Nanobody anti-PSA clone C12	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-Ricin C8	Research	N.A.	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[63]
Nanobody anti-Ricin D1	Research	N.A.	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[63]
Nanobody anti-SARS-CoV-1 clone 72	Research	N.A.	Pichia pastoris	Phage display	SBP Info	[60]
Nanobody anti-SARS-CoV-2 1B-3F-2A	Research	N.A.	Expi293 cells	Flow cytometry	SBP Info	[64]
Nanobody anti-SARS-CoV-2 3F-1B-2A	Research	N.A.	Expi293 cells	Flow cytometry	SBP Info	[64]
Nanobody anti-SARS-CoV-2 clone 21	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[65]
Nanobody anti-SARS-CoV-2 clone 23	Research	N.A.	Escherichia coli	Phage display and Ribosome display	SBP Info	[66]
Nanobody anti-SARS-CoV-2 clone H11-D4	Research	N.A.	Expi293 cells; Escherichia coli	Phage display	SBP Info	[67]
Nanobody anti-SARS-CoV-2 clone H11CH4	Research	N.A.	Expi293 cells; Escherichia coli	Phage display	SBP Info	[67]
Nanobody anti-SARS-CoV-2 clone Nb20	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[65]
Nanobody anti-SARS-CoV-2 matured clone 6	Research	Nb6	N.A.	Yeast display	SBP Info	[68]
Nanobody anti-SARS-CoV-2 Nanosota-1	Research	N.A.	Bacteria	Phage display	SBP Info	[69]
Nanobody anti-SARS-CoV-2 trivalent matured clone 6	Research	mNb6	N.A.	Yeast display	SBP Info	[68]
Nanobody anti-SARS-CoV-2 W25	Research	N.A.	Escherichia coli	Bacteria display	SBP Info	[70]
Nanobody anti-sbMATE clone 83	Research	N.A.	Escherichia coli BL21 (DE3)	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-sbMATE clone 86	Research	N.A.	Escherichia coli Shuffle T7	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-sbMATE clone 94	Research	N.A.	Escherichia coli BL21 (DE3)	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-SEB A3	Research	N.A.	Escherichia coli Rosetta (DE3)	Phage display	SBP Info	[63]
Nanobody anti-Survivin R7-A	Research	N.A.	Brevibacillus pBIC4	cDNA display	SBP Info	[72]
Nanobody anti-Survivin R7-B	Research	N.A.	Brevibacillus pBIC4	cDNA display	SBP Info	[72]
Nanobody anti-Survivin R7-C	Research	N.A.	Brevibacillus pBIC4	cDNA display	SBP Info	[72]
Nanobody anti-Survivin R7-D	Research	N.A.	Brevibacillus pBIC4	cDNA display	SBP Info	[72]
Nanobody anti-Survivin R7-E	Research	N.A.	Brevibacillus pBIC4	cDNA display	SBP Info	[72]
Nanobody anti-TGP Anti-TGP clone 68	Research	N.A.	Escherichia coli	Phage display	SBP Info	[73]
Nanobody anti-TGP clone 44	Research	GFP-special nanobody Enhancer	N.A.	Ribosome display; Phage display	SBP Info	[73]
Nanobody anti-TGP clone 66	Research	GFP-special nanobody Enhancer	N.A.	Ribosome display; Phage display	SBP Info	[73]
Nanobody anti-TGP clone 92	Research	GFP-special nanobody Enhancer	N.A.	Ribosome display; Phage display	SBP Info	[73]
Nanobody anti-TM287/288 clone 1	Research	N.A.	pSb_init	Phage display	SBP Info	[74]
Nanobody anti-TM287/288 clone 2	Research	N.A.	pSb_init	Phage display	SBP Info	[74]
Nanobody anti-VEGF	Research	N.A.	Escherichia coli WK6	Docking simulation experiments	SBP Info	[75]
Nanobody anti-VEGF clone 22	Research	N.A.	Escherichia coli	Phage display	SBP Info	[76]
Nanobody anti-VEGF clone 23	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[76]
Nanobody anti-VEGF clone 35	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[76]
Nanobody anti-VEGF clone 42	Research	N.A.	Escherichia coli WK6	Phage display	SBP Info	[76]
Nanobody anti-VEGFR-2 NTV1	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[77]
Nanobody anti-VEGFR-2 NTV2	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[77]
Nanobody anti-VEGFR-2 NTV3	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[77]
Nanobody anti-VEGFR-2 NTV4	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[77]
Nanobody anti-VGLUT1 clone 1	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 10	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 11	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 2	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 3	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 4	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 5	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 6	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 7	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 8	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VGLUT1 clone 9	Research	N.A.	Escherichia coli MC1061	Phage display	SBP Info	[78]
Nanobody anti-VSG cAbAn33-1-0-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-VSG cAbAn33-1-0-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-VSG cAbAn33-1-1-0	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-VSG cAbAn33-1-1-1	Research	cAbPSA-N8	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-AaHII NbAahII10	Research	N.A.	beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII NbAahII10C/A	Research	N.A.	Beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII NbAahII10C/S	Research	N.A.	Beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-AaHII NbAahII10C/T	Research	N.A.	Beta-D-thiogalactoside	N.A.	SBP Info	[34]
Nanobody anti-BC2T clone BC2	Research	N.A.	Escherichia coli	N.A.	SBP Info	[38]
Nanobody anti-ERBB1 EGa1	Research	N.A.	Escherichia coli	N.A.	SBP Info	[79]
Nanobody anti-GFP	Research	N.A.	Leishmania tarentolae	N.A.	SBP Info	[59]
Nanobody anti-HIF-1A clone 212	Research	N.A.	N.A.	Ribosome display	SBP Info	[56]
Nanobody anti-HIV-1 clone 2H10	Research	N.A.	Escherichia coli BL21 (DE3)	N.A.	SBP Info	[80]
Nanobody anti-KDELR2 clone 37	Research	N.A.	N.A.	Phage display and Ribosome display	SBP Info	[81], [15]
Nanobody anti-Lactose permease clone 9047	Research	N.A.	Escherichia coli	N.A.	SBP Info	[82]
Nanobody anti-mCherry LaM8-AK74	Research	LaM-8	N.A.	N.A.	SBP Info	[83]
Nanobody anti-mCherry LaM8-AK74-C450V	Research	LaM-8	N.A.	N.A.	SBP Info	[83]
Nanobody anti-mCherry LaM8-GG15	Research	LaM-8	N.A.	N.A.	SBP Info	[83]
Nanobody anti-mCherry LaM8-GG15-C450V	Research	LaM-8	N.A.	N.A.	SBP Info	[83]
Nanobody anti-PSA clone N8	Research	N.A.	Escherichia coli	N.A.	SBP Info	[39]
Nanobody anti-SARS-CoV-2 clone 11	Research	N.A.	N.A.	Yeast display	SBP Info	[84]
Nanobody anti-SARS-CoV-2 clone 18	Research	N.A.	N.A.	Yeast display	SBP Info	[84]
Nanobody anti-SARS-CoV-2 clone 3	Research	N.A.	N.A.	Yeast display	SBP Info	[84]
Nanobody anti-SARS-CoV-2 clone 6	Research	N.A.	N.A.	Yeast display	SBP Info	[68]
Nanobody anti-sbMATE clone 80	Research	N.A.	N.A.	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-sbMATE clone 81	Research	N.A.	N.A.	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-sbMATE clone 93	Research	N.A.	N.A.	mRNA display; cDNA display	SBP Info	[71]
Nanobody anti-TmrAB clone 9F10	Research	N.A.	Escherichia coli TG1	N.A.	SBP Info	[85]
Nanobody anti-GFP cAbGFP4	Research	N.A.	N.A.	N.A.	SBP Info	[86]
Nanobody anti-GFP enhancer	Research	N.A.	N.A.	N.A.	SBP Info	[87]
Nanobody anti-GFP minimizer	Research	N.A.	N.A.	N.A.	SBP Info	[87]
Nanobody anti-SARS-CoV-2 clone 16	Research	N.A.	N.A.	N.A.	SBP Info	[88]
Nanobody anti-SARS-CoV-2 clone 45	Research	N.A.	N.A.	N.A.	SBP Info	[88]
Nanobody anti-SARS-CoV-2 clone 68	Research	N.A.	N.A.	N.A.	SBP Info	[88]
Nanobody anti-SARS-CoV-2 clones	Research	N.A.	N.A.	N.A.	SBP Info	[89]
Nanobody anti-SARS-CoV-2 LR1	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SARS-CoV-2 LR5	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SARS-CoV-2 MR17	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SARS-CoV-2 MR3	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SARS-CoV-2 MR4	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SARS-CoV-2 SR4	Research	N.A.	N.A.	N.A.	SBP Info	[90]
Nanobody anti-SLC26Dg clone 5776	Research	N.A.	N.A.	N.A.	SBP Info	[91]

References

1	Caplacizumab for Acquired Thrombotic Thrombocytopenic Purpura. N Engl J Med. 2016 Feb 11;374(6):511-22.
2	Novel aptamer to von Willebrand factor A1 domain (TAGX-0004) shows total inhibition of thrombus formation superior to ARC1779 and comparable to caplacizumab. Haematologica. 2020 Nov 1;105(11):2631-2638.
3	The thermal stability of immunoglobulin: unfolding and aggregation of a multi-domain protein. Biophys J. 2000 Jan;78(1):394-404.
4	Inflammation and joint destruction may be linked to the generation of cartilage metabolites of ADAMTS-5 through activation of toll-like receptors. Osteoarthritis Cartilage. 2020 May;28(5):658-668.
5	Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody. Cell. 2019 Jan 24;176(3):479-490.e12.
6	Angiotensin and biased analogs induce structurally distinct active conformations within a GPCR.Science. 2020 Feb 21;367(6480):888-892.
7	L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction. Nat Commun. 2019 Apr 18;10(1):1807.
8	Molecular basis for high affinity agonist binding in GPCRsScience. 2019 May 24;364(6442):775-778.
9	An Ultrahigh-Affinity Complement C4b-Specific Nanobody Inhibits In Vivo Assembly of the Classical Pathway Proconvertase. J Immunol. 2020 Sep 15;205(6):1678-1694.
10	Identification and characterization of blocking nanobodies against human CD70. Acta Biochim Biophys Sin (Shanghai). 2022 Oct 25;54(10):1518-1527.
11	Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca na?ve phage display library. J Nanobiotechnology. 2022 May 14;20(1):231.
12	Inhibition of cleavage of human complement component C5 and the R885H C5 variant by two distinct high affinity anti-C5 nanobodies. J Biol Chem. 2023 Aug;299(8):104956.
13	Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling. Elife. 2020 Aug 7;9:e58464.
14	Structure of an AMPK complex in an inactive, ATP-bound state. Science. 2021 Jul 23;373(6553):413-419.
15	Synthetic single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7:e34317.
16	Structural characterization of a novel GPVI-nanobody complex reveals a biologically active domain-swapped GPVI dimer. Blood. 2021 Jun 17;137(24):3443-3453.
17	Structural insights into the human D1 and D2 dopamine receptor signaling complexes. Cell. 2021 Feb 18;184(4):931-942.e18.
18	Crystal structure of the 2 adrenergic receptor-Gs protein complex. Nature. 2011 Jul 19;477(7366):549-55.
19	IgG-Binding Nanobody Capable of Prolonging Nanobody-Based Radiotracer Plasma Half-Life and Enhancing the Efficacy of Tumor-Targeted Radionuclide Therapy. Bioconjug Chem. 2022 Jul 20;33(7):1328-1339.
20	A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19. 2021
21	A potent synthetic nanobody with broad-spectrum activity neutralizes SARS-CoV-2 virus and the Omicron variant BA.1 through a unique binding mode. J Nanobiotechnology. 2022 Sep 15;20(1):411.
22	Structures of synthetic nanobody-SARS-CoV-2-RBD complexes reveal distinct sites of interaction and recognition of variants. Res Sq. 2021 Jun 16;rs.3.rs-625642.
23	A high-affinity RBD-targeting nanobody improves fusion partner's potency against SARS-CoV-2. PLoS Pathog. 2021 Mar 3;17(3):e1009328.
24	An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction. Nat Commun. 2020 Sep 4;11(1):4420.
25	Protein Data Bank. (7CAN)Structure of sybody MR17-K99Y in complex with the SARS-CoV-2 S Receptor-binding domain (RBD)
26	A Potent Neutralizing Nanobody Targeting the Spike Receptor-Binding Domain of SARS-CoV-2 and the Structural Basis of Its Intimate Binding. Front Immunol. 2022 May 18;13:820336.
27	Development of a bispecific nanobody conjugate broadly neutralizes diverse SARS-CoV-2 variants and structural basis for its broad neutralization. PLoS Pathog. 2023 Nov 30;19(11):e1011804.
28	A Na?ve Phage Display Library-Derived Nanobody Neutralizes SARS-CoV-2 and Three Variants of Concern. Int J Nanomedicine. 2023 Oct 16;18:5781-5795.
29	NeutrobodyPlex-monitoring SARS-CoV-2 neutralizing immune responses using nanobodies. EMBO Rep. 2021 May 5;22(5):e52325.
30	Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates. PLoS One. 2016 Feb 19;11(2):e0149393.
31	Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol. 2017 Jun 26;2:17103.
32	Viral GPCR US28 can signal in response to chemokine agonists of nearly unlimited structural degeneracy. Elife. 2018 Jun 8;7:e35850.
33	Cryo-EM structures of the caspase-activated protein XKR9 involved in apoptotic lipid scrambling. Elife. 2021 Jul 15;10:e69800.
34	Development of Cys38 knock-out and humanized version of NbAahII10 nanobody with improved neutralization of AahII scorpion toxin. Protein Eng Des Sel. 2011 Sep;24(9):727-35.
35	Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nat Struct Mol Biol. 2018 Mar;25(3):289-296.
36	Synthetic nanobodies as angiotensin receptor blockers. Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20284-20291.
37	Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach. J Biomol NMR. 2019 Jul;73(6-7):375-384.
38	Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy. Sci Rep. 2016 Jan 21;6:19211.
39	Disulfide bond introduction for general stabilization of immunoglobulin heavy-chain variable domains. J Mol Biol. 2008 Mar 21;377(2):478-88.
40	Structural basis of nanobody-mediated blocking of BtuF, the cognate substrate-binding protein of the Escherichia coli vitamin B12 transporter BtuCD. Sci Rep. 2017 Oct 30;7(1):14296.
41	Surface proteomics reveals CD72 as a target for in vitro-evolved nanobody-based CAR-T cells in KMT2A/MLL1-rearranged B-ALL. Cancer Discov. 2021 Mar 16;candisc.0242.2020.
42	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies. Nat Commun. 2019 Jun 14;10(1):2636.
43	Phage-Mediated Immuno-PCR for Ultrasensitive Detection of Cry1Ac Protein Based on Nanobody. J Agric Food Chem. 2016 Oct 19;64(41):7882-7889.
44	Structural Characterization of Humanized Nanobodies with Neutralizing Activity against the Bordetella pertussis CyaA-Hemolysin: Implications for a Potential Epitope of Toxin-Protective Antigen. Toxins (Basel). 2016 Apr 1;8(4):99.
45	Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport. Nat Struct Mol Biol. 2014 Nov;21(11):990-6.
46	Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1. J Am Chem Soc. 2019 Feb 13;141(6):2404-2412.
47	Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT 3 receptor via a common vestibule site. Elife. 2020 Jan 28;9:e51511.
48	A lipid site shapes the agonist response of a pentameric ligand-gated ion channel. Nat Chem Biol. 2019 Dec;15(12):1156-1164.
49	Structural basis for GABA A receptor potentiation by neurosteroids. Nat Struct Mol Biol. 2017 Nov;24(11):986-992.
50	A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec;11(12):1253-60.
51	Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery. Protein Sci. 2016 Jun;25(6):1129-37.
52	Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 2010 Dec;19(12):2389-401.
53	Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein. Microb Cell Fact. 2017 Dec 12;16(1):223.
54	Identification of nanobodies against hepatocellular carcinoma marker glypican-3. Mol Immunol. 2021 Mar;131:13-22.
55	An ER-directed gelsolin nanobody targets the first step in amyloid formation in a gelsolin amyloidosis mouse model. Hum Mol Genet. 2015 May 1;24(9):2492-507.
56	In vitro affinity maturation to improve the efficacy of a hypoxia-inducible factor 1 single-domain intrabody. Biochem Biophys Res Commun. 2020 Sep 3;529(4):936-942.
57	The ABC exporter IrtAB imports and reduces mycobacterial siderophores. Nature. 2020 Apr;580(7803):413-417.
58	Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425.
59	A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein-Protein Interactions. Int J Mol Sci. 2017 Dec 11;18(12):2681.
60	Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell. 2020 Jun 11;181(6):1436-1441.
61	Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13386-91.
62	Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody. Sci Rep. 2017 Apr 19;7:46641.
63	Contributions of the complementarity determining regions to the thermal stability of a single-domain antibody. PLoS One. 2013 Oct 15;8(10):e77678.
64	Development of humanized tri-specific nanobodies with potent neutralization for SARS-CoV-2. Sci Rep. 2020 Oct 20;10(1):17806.
65	Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2. Science. 2020 Dec 18;370(6523):1479-1484.
66	Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2. Nat Commun. 2020 Nov 4;11(1):5588.
67	Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Nat Struct Mol Biol. 2020 Sep;27(9):846-854.
68	An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike. Science. 2020 Dec 18;370(6523):1473-1479.
69	The Development of a Novel Nanobody Therapeutic for SARS-CoV-2.bioRxiv. 2020 Nov 17;2020.11.17.386532.
70	Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody. Sci Rep. 2021 Feb 8;11(1):3318.
71	Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor. Sci Rep. 2017 Dec 21;7(1):17996.
72	Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display. Biochem Biophys Res Commun. 2018 Sep 10;503(3):2054-2060.
73	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Commun Biol. 2020 Dec 10;3(1):753.
74	The extracellular gate shapes the energy profile of an ABC exporter. Nat Commun. 2019 May 21;10(1):2260.
75	A nanobody-derived mimotope against VEGF inhibits cancer angiogenesis. J Enzyme Inhib Med Chem. 2020 Dec;35(1):1233-1239.
76	Inhibition of angiogenesis in human endothelial cell using VEGF specific nanobody. Mol Immunol. 2015 May;65(1):58-67.
77	Generation and characterization of a human nanobody against VEGFR-2. Acta Pharmacol Sin. 2016 Jun;37(6):857-64.
78	Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport. Biochemistry. 2017 Aug 1;56(30):3962-3971.
79	Capillary electrophoresis-based assessment of nanobody affinity and purity. Anal Chim Acta. 2014 Mar 25;818:1-6.
80	Structure of HIV-1 gp41 with its membrane anchors targeted by neutralizing antibodies. Elife. 2021 Apr 19;10:e65005.
81	Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor. Science. 2019 Mar 8;363(6431):1103-1107.
82	Crystal Structure of a ligand-bound LacY-Nanobody Complex. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8769-8774.
83	Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun. 2020 Aug 13;11(1):4044.
84	An ultra-high affinity synthetic nanobody blocks SARS-CoV-2 infection by locking Spike into an inactive conformation. bioRxiv. 2020 Aug 10;2020.08.08.238469.
85	Conformation space of a heterodimeric ABC exporter under turnover conditions. Nature. 2019 Jul;571(7766):580-583.
86	Broad-Spectrum Proteome Editing with an Engineered Bacterial Ubiquitin Ligase Mimic. ACS Cent Sci. 2019 May 22;5(5):852-866.
87	Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol. 2010 Jan;17(1):133-8.
88	Synthetic nanobody-SARS-CoV-2 receptor-binding domain structures identify distinct epitopes. bioRxiv. 2021 Jan 27;2021.01.27.428466.
89	Highly potent, stable nanobodies stop SARS-CoV-2
90	A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection.Nat Commun. 2021 Jul 30;12(1):4635.
91	Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family. Nat Struct Mol Biol. 2015 Oct;22(10):803-8.