General Information of Synthetic Binding Protein (SBP) (ID: SBP003468)
SBP Name
Nanobody anti-TssK nbK27
Synonyms
nbK27
Molecular Weight 12.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
PDB ID 5MWN
Sequence Length 119
SBP Sequence
>Nanobody anti-TssK K27
QVQLVESGGGLVQPGGSLRLSCAASGIMLGYFTMAWYRQAPGKQRELVATEISGGSANYA
DAVKGRFTISRDNARSTVYLQMNSLKPEDTAVYYCDARIWRGTVYDNISGPGTQVTVSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Type VI secretion protein
BTS Info
Binder Research tool N.A. Aix-Marseille University [1]
References
1 Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol. 2017 Jun 26;2:17103.