General Information of Synthetic Binding Protein (SBP) (ID: SBP003408)
SBP Name
Nanobody anti-BC2T clone BC2
Synonyms
Nb BC2
Molecular Weight 13.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
PDB ID 5IVO; 5IVN
Sequence Length 125
SBP Sequence
>Nanobody anti-BC2T clone BC2
QVQLVESGGGLVQPGGSLTLSCTASGFTLDHYDIGWFRQAPGKEREGVSCINNSDDDTYY
ADSVKGRFTIFMNNAKDTVYLQMNSLKPEDTAIYYCAEARGCKRGRYEYDFWGQGTQVTV
SSKKK
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Catenin beta-1
BTS Info
Binder Research tool Kd: 1.4 nM Eberhard-Karls University Tuebingen [1]
References
1 Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy. Sci Rep. 2016 Jan 21;6:19211.