General Information of Synthetic Binding Protein (SBP) (ID: SBP003364)
SBP Name
Nanobody anti-CyaA-Hly clone 2
Synonyms
VHH2
Molecular Weight 13.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 125
SBP Sequence
>Nanobody anti-CyaA-Hly clone 2
VQLVESGGGSVQAGGSLRLSCTVSGYTDNRYCMGWFRQAPGKEREGVAGINDFGGTTYYV
DSVKGRFTISQNNAKNTVYLQMNSLKPEDTAIYYCAIAPRIWGSICSPGTQYNYWGQGTM
VTVSS
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Bifunctional hemolysin/adenylate cyclase
BTS Info
Neutralizer Pertussis [ICD-11: 1C12.Z] N.A. Mahidol University [1]
References
1 Structural Characterization of Humanized Nanobodies with Neutralizing Activity against the Bordetella pertussis CyaA-Hemolysin: Implications for a Potential Epitope of Toxin-Protective Antigen. Toxins (Basel). 2016 Apr 1;8(4):99.