General Information of Synthetic Binding Protein (SBP) (ID: SBP003130)
SBP Name
Monobody anti-Gdx-Clo L10
Synonyms
Monobody L10
Molecular Weight 9.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display and yeast display
Highest Status Research
PDB ID 6WK5
Sequence Length 91
SBP Sequence
>Monobody anti-Gdx-Clo L10
VSSVPTKLEVVAATPTSLLISWDAGHWWEWVTYYRITYGETGGNSPVQEFTVPGYSSTAT
ISGLKPGVDYTITVYAPTSDYGSPISINYRT
3D Structure
Experimentally Validated Structure
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Template Name PDBID 5NKQ
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Small multidrug resistance family transporter Gdx-Clo
BTS Info
Binder Tools for solving crystal structure of Gdx-Clo N.A. University of Michigan [1]
References
1 The structural basis of promiscuity in small multidrug resistance transporters. Nat Commun. 2020 Nov 27;11(1):6064.