General Information of Synthetic Binding Protein (SBP) (ID: SBP003101)
SBP Name
scFv anti-FL-bio 4M5.3
Synonyms
scFv 4M5.3
Molecular Weight 29.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Yeast display
Highest Status Research
PDB ID 1X9Q
Sequence Length 268
SBP Sequence
>scFv anti-FL-bio 4 M5.3
AARPDYKDDDDKASDVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLRWYLQKPG
QSPKVLIYKVSNRVSGVPDRFSGSGSGTDFTLKINRVEAEDLGVYFCSQSTHVPWTFGGG
TKLEIKSSADDAKKDAAKKDDAKKDDAKKDGGVKLDETGGGLVQPGGAMKLSCVTSGFTF
GHYWMNWVRQSPEKGLEWVAQFRNKPYNYETYYSDSVKGRFTISRDDSKSSVYLQMNNLR
VEDTGIYYCTGASYGMEYLGQGTSVTVS
3D Structure
Experimentally Validated Structure
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Template Name Fv 4-4-20
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Fluorescein-biotin
BTS Info
Binder Research tool Kd: 0.000048 nM University of Illinois at Chicago [1]
References
1 Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10701-5.