General Information of Synthetic Binding Protein (SBP) (ID: SBP001803)
SBP Name
scFv anti-TPO T5
Synonyms
scFv T5
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli HB2151
Selection Method Phage display
Highest Status Research
SBP Sequence
>VH Chain
QVQLVQSGAEVKKPGASMKVSCKASEFTFTHYDINWVRQATGQGLEWMGWMNPNSGRTGY
GQNFQGRVTITRNSSTSTIYMELSSLISEDTAVYYCVRNSKAFRALEIWGQGTMVTVSS
>VL Chain
EIVLTHPPSTLSASIGDRVTITCRASQSVTQWLAWYQQKPGKAPRLLIYKASHLHDGVPS
RFSGSGSGTEFTLTINSLQPDDFATPYCQQYYTYPYT FGQGTKLEIKR
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Thyroid peroxidase
BTS Info
Binder Autoimmune thyroiditis [ICD-11: 5A03.2] Kd: 0.77 nM National Center for scientific research - joint research group 5094 [1]
References
1 Thyroid peroxidase autoantibodies obtained from random single chain FV libraries contain the same heavy/light chain combinations as occur in vivo. Endocrinology. 2001 Nov;142(11):4740-50.