General Information of Synthetic Binding Protein (SBP) (ID: SBP001751)
SBP Name
scFv anti-fluorescein 3M
Synonyms
scFv 3M
Molecular Weight 27.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 251
SBP Sequence
>scFv anti-fluorescein 3M
DIQMIQSPSSLSASVGDRVTITCRASQSLVRSQGNTYLRWYQQKPGKAPKVLIYKVSNRV
SGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSSHVPWTFGQGTKVELKRATPSHNS
HQVPSAGGPTANGSEVQLVESGGGLVQPGGSLRLSCAASGFTFSDYWMNWVRQAPGKGLE
WVAQIRNKPYNYETYYADSVKGRFTISRDTSKNTVYLQMNSLREDTAVYYCTGSYYGMDY
WGQGTLVTVSS
3D Structure
Computationally Modelled Structure
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Template Name scFv 4D5Flu
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Fluorescein
BTS Info
Binder Tools as a reagent with well folding and stable properties Kd: 1.2 nM Biochemical Institute of the University of Zurich [1]
References
1 Selection for improved protein stability by phage display. J Mol Biol. 1999 Nov 19;294(1):163-80.