Details of the SBP
| General Information of Synthetic Binding Protein (SBP) (ID: SBP001681) | ||||||
|---|---|---|---|---|---|---|
| SBP Name |
scFv anti-Fluorescein B5-2
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| Synonyms |
scFv B5-2
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| Design Method | Traditional methods (Site-directed mutagenesis and/or Directed evolution) | |||||
| Expression System | Escherichia coli | |||||
| Selection Method | Ribosome display and In vitro diversification by DNA shuffling | |||||
| Highest Status | Research | |||||
| SBP Sequence |
>VH Chain
EVQLQQSGPELAKPGASVKISCKATGYAFSSYRIEWVRQRPGQGLEWIGVINPGSGGTNY NEKFKGKAALTANKSSSTAYMQLSSLTSEDSAVYYCARRGNYYFGYWGQGTTLTVSL >VL Chain DIVMTQSQRFMSTSVGDRVSVTCKASQSVGTNVAWYQQKPGQSPKALIHSASYRYSGVPD RFTGSGSGTDFTLTISDVQSEDLAEYFCRQYNSYPWTFGGGTKLELKR |
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| Template Name | scFv 12 | |||||
| Template Sequence |
>VH Chain
EVQLQQSGPELVKPGASVKISCKATGYAFSSYRIEWVKQRPGQGLEWIGVINPGSGGTNY NEKFKGKAALTADKSSSTAYMQLSSLTSEDSAVYYCARRGNYYFDYWGQGTTLTVSS >VL Chain DIVMTQSQKFMSTSVGDRVSVTCKASQNVDTNVAWYQQKPGQSPKALIHSASYRYSGVPD RFTGSGSGTDFTLTISNVQSEDLAEYFCRQYNSHPWTFGGGTKLELKR |
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| Protein Scaffold Information of This SBP | ||||||
|---|---|---|---|---|---|---|
| Scaffold ID | PS057 | [1] | ||||
| Scaffold Name | scFv | |||||
| Scaffold Class | Antibody fragment | |||||
| Fold Type | Beta-Sheets + Loops | |||||
| Binding Target(s) of This SBP (BTS) |
|---|
| BTS Name | Details | Mechanism | Application | Affinity | Research Organization | Ref |
|---|---|---|---|---|---|---|
| Fluorescein | Binder | Research tool | N.A. | University of Zurich | [1] | |