Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001667)
SBP Name	scFv anti-PA 14B7
Synonyms	scFv 14B7
Molecular Weight	26.1 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli
Selection Method	APEx two-hybrid
Highest Status	Research
Sequence Length	247
SBP Sequence	>scFv anti-PA 14B7 DIVLIQSTSSLSASLGDRVTISCRASQDIRNYLNWYQQKPDGTVKLLIYYTSRLQSGVPS RFSGSGSGTDYSLTISNLEQEDIGTYFCQQGNTLPWTFGGGTKLEIKRGGGGSGGGGSGG GGSGGGGSEVQLQQSGPELVKPGASVKISCKDSGYAFSSSWMNWVKQRPGQGPEWIGRIY PGDGDTNYNGKFKGKATLTADKSSSTAYMQLSSLTSVDSAVYFCARSGLLRYAMDYWGQG TSVTVSS
3D Structure
	Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID	PS057 Scaffold Info					[1]
Scaffold Name	scFv
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Protective antigen	BTS Info	Binder	Research tool	Kd: 3.3 nM	University of Texas	[1]

References

1

APEx 2-hybrid, a quantitative protein-protein interaction assay for antibody discovery and engineering. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8247-52.