Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001623)
SBP Name	scFv anti-GA-pyridine 73MuL-V94A
Synonyms	scFv 73MuL-V94A
Molecular Weight	26.2 kDa
Thermal Denaturation TEMP	62.7 ℃
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli
Selection Method	Phage display
Highest Status	Research
Sequence Length	242
SBP Sequence	>scFv anti-GA-pyridine 73MuL-V94A QVKLQQSGPSLVKPSQTLSLTCSVTGDSITSGYWNWIRKFPGNKFEYLGYISYSGRTYYN PSLKSRISITRDTSKNQYYLQLNSVTTEDTATYYCSRPYYRYDYAIDYWGQGTTVTVSSG GGGSGGGGSGGGGSDIELTQSPAIMSASLGERVTMTCTASSSVSSSYLHWYQQKPGSSPK LWIYSTSNLASGVPARFSSSGSGTSYSLTISRMEAEDAATYYCQQSRKAPYTFGGGTKLE IK
3D Structure
	Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID	PS057 Scaffold Info					[1]
Scaffold Name	scFv
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
3-hydroxy-4-hydroxymethyl-1-(5-amino-5-carboxypentyl) pyridinium cation	BTS Info	Binder	Tools for reducing inter-domain motion	Kd: 149 nM	Kumamoto University	[1]

References

1

Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End-Product (AGE), with Reduced Inter-Domain Motion. Molecules. 2017 Oct 10;22(10):1695.