General Information of Synthetic Binding Protein (SBP) (ID: SBP001615)
SBP Name
scFv anti-IL-17A 8D3
Synonyms
scFv 8D3
Thermal Denaturation TEMP 59.4 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Yeast display
Highest Status Research
SBP Sequence
>VH Chain
QVQLQQSGPGLVKPPQTLSLTCAISGDSVSSNSAAWDWIRQSPSRGLEWLGRTYYRSKWS
HDYAVSVKNRITINPDTSKNQFSLQLNSVSPEDTAVYYCVREGDYGDYGYWGQGTLVTVS
S
>VL Chain
SSELTQDPAVSVALGQTVRITCQGDSLRGYYASWYQQKPGQAPVLVIYYDDDRPTGIPER
FSGSNSGNSATLTISRVEAGDEADYYCAAWDVSLNGPVFGGGTKVTVLS
Template Name scFv 7H9
Template Sequence
>VH Chain
QVQLQQSGPGLVKPSQTLSLTCAISGDSVSSNSAAWNWIRQSPSRGLEWLGRTYYRSKWS
HDYAVSVKSRITINPDTSKNQFSLQLNSVTPEDTAVYYCAREGEYGDYGYWGQGTLVTVS
S
>VL Chain
SSELTQDPAVSVALGQTVRITCQGDSLRSYYASWYQQKPGQAPVLVIYYDDDRPTGIPER
FSGSNSGNTATLTISRVEAGDEADYYCAAWDDSLNGPVFGGGTKVTVLS
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Interleukin-17A
BTS Info
Binder Autoimmune disease [ICD-11: 4A4Z]; Cancers [ICD-11: 2D4Z] Kd: 0.47 nM Chinese Academy of Medical Sciences & Peking Union Medical College [1]
References
1 Improvement in affinity and thermostability of a fully human antibody against interleukin-17A by yeast-display technology and CDR grafting. Acta Pharm Sin B. 2019 Sep;9(5):960-972.