Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001528)
SBP Name
Fab hu17E8
Synonyms
hu17E8
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
SBP Sequence
>VH Chain
EVQLQQSGPELVKPGASVRMSCKSSGYIFTDFYMNWVRQSHGKSLDYIGYISPYSGVTGY
NQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAGSSGNKWAMDYWGHGASVTVSSA
KTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDL
YTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP
>VL Chain
DVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLNWYLQKAGQSPKLLIYKVSNRF
SGVPDRFSGSGSGTDFTLKISRVEAEDLGIYFCSQTTHVPPTFGGGTKLEIKRADAAPTV
SIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSM
SSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC
Protein Scaffold Information of This SBP
Scaffold ID PS034
Scaffold Info
[1] , [2]
Scaffold Name Fab
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Phenyl[1-pentyl]phosphonate
BTS Info
Binder Tools for optimizing affinity for transition-state analog?binding N.A. Genentech Inc [1] , [2]
References
1 Phage display of a catalytic antibody to optimize affinity for transition-state analog binding. Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10063-8.
2 Antibody humanization using monovalent phage display. J Biol Chem. 1997 Apr 18;272(16):10678-84.