General Information of Synthetic Binding Protein (SBP) (ID: SBP001286)
SBP Name
Monobody anti-MAPK p38 alpha clone alpha-p38mb
Synonyms
Monobody alpha-p38mb
Molecular Weight 10.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Yeast display
Highest Status Research
Sequence Length 95
SBP Sequence
>Monobody anti-MAPK p38 alpha clone alpha-p38mb
VSDVPRDLEVVAATPTSLLISWDPPRCRRARYYRITYGETGGNSPVQEFTVPGSKSTATI
SGLKPGVDYTITVYAVTSMPRLGIPRKSISINYRT
3D Structure
Computationally Modelled Structure
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Template Name Monobody 674mb
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Mitogen-activated protein kinase 14
BTS Info
Binder Research tool Kd: 649 nM University of North Carolina at Chapel Hill [1]
References
1 Engineering a Protein Binder Specific for p38 with Interface Expansion. Biochemistry. 2018 Jul 31;57(30):4526-4535.