Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001282)
SBP Name
Monobody anti-Integrin FNfn10-3JCLI4
Synonyms
Monobody FNfn10-3JCLI4
Molecular Weight 10.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 101
SBP Sequence
>Monobody anti-Integrin FNfn10-3JCLI4
VSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATIS
GLKPGVDYTITVYAVTPRGDWNEGSKPISINYRTEIDKPSQ
3D Structure
Computationally Modelled Structure
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Template Name 10th domain of fibronectin type III (10Fn3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1] , [2]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Integrin alpha-v-beta-3
BTS Info
Binder Cancers [ICD-11: 2D4Z] Kd: 0.8 nM University of North Carolina at Chapel Hill; University of Rochester Medical Center [1] , [2]
References
1 Fibronectin type III domain based monobody with high avidity. Biochemistry. 2007 Nov 6;46(44):12656-64.
2 Engineered fibronectin type III domain with a RGDWXE sequence binds with enhanced affinity and specificity to human alphavbeta3 integrin. J Mol Biol. 2003 Mar 7;326(5):1475-88.