General Information of Synthetic Binding Protein (SBP) (ID: SBP001280)
SBP Name
Monobody anti-Erk-2 F7.8
Synonyms
Monobody F7.8
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Human embryonic kidney?cells 293
Selection Method Yeast display
Highest Status Research
SBP Sequence
>BC Loop
HPRPCSSY
>DE Loop
FASS
>FG Loop
RRSLSW
Template Name 10th domain of fibronectin type III (10Fn3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Mitogen-activated protein kinase 1
BTS Info
Inhibitor Research tool Kd: 1.3 nM University at Buffalo [1]
References
1 Epitope-guided engineering of monobody binders for in vivo inhibition of Erk-2 signaling. ACS Chem Biol. 2013 Mar 15;8(3):608-16.