General Information of Synthetic Binding Protein (SBP) (ID: SBP001244)
SBP Name
Monobody anti-R-PE dye PEbody
Synonyms
Monobody PEbody
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Saccharomyces cerevisiae
Selection Method Yeast display and Fluorescence-activated cell sorting
Highest Status Research
Template Name Monobody S4
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
R-Phycoerythrin dye
BTS Info
Binder Tools as FRET biosensor assembly and imaging at live-cell surface Kd: 5.7 nM University of California [1]
References
1 Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface. Cell Chem Biol. 2018 Apr 19;25(4):370-379.e4.