General Information of Synthetic Binding Protein (SBP) (ID: SBP001238)
SBP Name
Monobody anti-BgaD-D clone S10
Synonyms
Monobody BgaD_S10
Molecular Weight 9.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display and yeast display
Highest Status Research
Sequence Length 92
SBP Sequence
>Monobody anti-BgaD-D clone S10
VSSVPTKLEVVAATPTSLLISWDAPAVTVVFYLITYGETGASSWPGYQEFTVPGSKSTAT
ISGLKPGVDYTITVYAQYPWDTGSPISINYRT
3D Structure
Computationally Modelled Structure
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Template Name 10th domain of fibronectin type III (10Fn3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
BgaD-D
BTS Info
Binder Tools for alteration of enzyme specificity Kd: 15 nM University of Chicago [1]
References
1 Monobody-mediated alteration of enzyme specificity. Nat Chem Biol. 2015 Oct;11(10):762-4.