General Information of Synthetic Binding Protein (SBP) (ID: SBP001223)
SBP Name
Monobody anti-Fluoride clone X1
Synonyms
Monobody X1
Molecular Weight 9.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 6D0J
Sequence Length 93
SBP Sequence
>Monobody anti-Fluoride clone X1
GSVSSVPTKLEVVAATPTSLLISWDASSSSVSYYRITYGETGGNSPVQEFTVPGSSSTAT
ISGLSPGVDYTITVYAHGWLQWYMSPISINYQT
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
CLC-type fluoride
BTS Info
Binder Research tool N.A. Brandeis University [1]
References
1 A CLC-type F -/H + antiporter in ion-swapped conformations. Nat Struct Mol Biol. 2018 Jul;25(7):601-606.