General Information of Synthetic Binding Protein (SBP) (ID: SBP001085)
SBP Name
Nanobody anti-BamA F7
Synonyms
NanoF7
Molecular Weight 16.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli MC1061
Selection Method Phage display
Highest Status Research
Sequence Length 152
SBP Sequence
>Nanobody anti-BamA F7
GSSSQVQLVESGGGLVQAGGSLRLSCAASGRTFSRYAMAWFRRAPGKEREFVAAISASAG
TIFYTDSVKGRFTISRDHAKNTVSLQMNSLRPEDTAVYYCAAKTGTWATLDRRYDYWGQG
TRVTVSAGRAGEQKLISEEDLNSAVDHHHHHH
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Outer membrane protein assembly factor BamA
BTS Info
Binder Research tool Kd: 1.8 nM University of Basel [1]
References
1 Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach. J Biomol NMR. 2019 Jul;73(6-7):375-384.