General Information of Synthetic Binding Protein (SBP) (ID: SBP001083)
SBP Name
Nanobody anti-BamA E6
Synonyms
NanoE6
Molecular Weight 16.5 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli MC1061
Selection Method Phage display
Highest Status Research
PDB ID 6QGW
Sequence Length 145
SBP Sequence
>Nanobody anti-BamA E6
GSSSQMQLVESGGGLVQAGGSLTLSCAASGRTFSDYDMGWFRQAPGKAREFVARISRSGR
MTSLADSVKGRFTISRDNGKRTVYLQMNSLKPEDTAVYYCAADPQWSRVRSGADYWGQGT
RVTVSAGRAGEQKLISEEDLNSAVDHHHHHH
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Outer membrane protein assembly factor BamA
BTS Info
Binder Research tool Kd: 5.5 nM University of Basel [1]
References
1 Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach. J Biomol NMR. 2019 Jul;73(6-7):375-384.