General Information of Synthetic Binding Protein (SBP) (ID: SBP001070)
SBP Name
Nanobody anti-PglK clone 87
Synonyms
Nb87
Molecular Weight 13.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli WK6
Selection Method Phage display
Highest Status Research
PDB ID 5NBD
Sequence Length 123
SBP Sequence
>Nanobody anti-PglK clone 87
QVQLQESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAITSGGGSTSY
SDAVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAKGYVVDFLDLAEYDSWGQGTQVT
VSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
ABC-type lipopolysaccharide transporter PglK
BTS Info
Inhibitor Research tool N.A. ETH Zurich [1]
References
1 Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody. Sci Rep. 2017 Apr 19;7:46641.