General Information of Synthetic Binding Protein (SBP) (ID: SBP001059)
SBP Name
Nanobody anti-DMT clone 6616
Synonyms
Nb6616
Molecular Weight 13.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli MC1061
Selection Method Phage display
Highest Status Research
PDB ID 5M94
Sequence Length 120
SBP Sequence
>Nanobody anti-DMT clone 6616
VQLQESGGGLVQAGGSLRLSCAASRSIFSIDTANWYRQPPGMQRELVATITRDGNANYAD
SVKGRFTISRDRARNTVYLQMNSLKPEDTGVYYCNAAIRTTVRTSAQEYWGQGTQVTVSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
DMT family transporter
BTS Info
Binder Research tool Kd: 37000 nM University of Zurich [1]
References
1 Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport. Nat Struct Mol Biol. 2014 Nov;21(11):990-6.