General Information of Synthetic Binding Protein (SBP) (ID: SBP001047)
SBP Name
Nanobody anti-TGP clone 44
Synonyms
Sb44
Molecular Weight 12.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Ribosome display; Phage display
Highest Status Research
Sequence Length 114
SBP Sequence
>Nanobody anti-TGP clone 44
QVQLVESGGGLVQAGGSLRLSCAASGFPVGRASMWWYRQAPGKEREWVAAISSYGWVTAY
ADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCEVSVGTGYRGQGTQVTVS
3D Structure
Computationally Modelled Structure
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Template Name GFP-special nanobody Enhancer
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent GFP-like protein
BTS Info
Binder Research tool Kd: 4.4 nM University of Zurich [1]
References
1 An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Commun Biol. 2020 Dec 10;3(1):753.