General Information of Synthetic Binding Protein (SBP) (ID: SBP000891)
SBP Name
RPtag anti-PDGFRbeta mutant
Synonyms
RPtag(large) H122L, A253R mutant
Molecular Weight 28.1 kDa
Thermal Denaturation TEMP 102 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Highest Status Research
Sequence Length 265
SBP Sequence
>RPtag anti-PDGFRbeta mutant
SQDPNSSSMKEGKTIGLVISTLNNPFFVTLKNGAEEKAKELGYKIIVEDSQNDSSKELSN
VEDLIQQKVDVLLINPVDSDAVVTAIKEANSKNIPVITIDRSANGGDVVSLIASDNVKGG
EMAAEFIAKALKGKGNVVELEGIPGASAARDRGKGFDEAIAKYPDIKIVAKQAADFDRSK
GLSVMENILQAQPKIDAVFAQNDEMALGAIKAIEAANRQGIIVVGFDGTEDALKAIKEGK
MRATIAQQPALMGSLGVEMADKYLK
3D Structure
Computationally Modelled Structure
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Template Name RPtag(Large)
Protein Scaffold Information of This SBP
Scaffold ID PS056
Scaffold Info
[1]
Scaffold Name RPtag
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Platelet-derived growth factor receptor beta
BTS Info
Binder Research tool Kd: 15000 nM Ichor Therapeutics, Inc; RecombiPure, Inc [1]
References
1 RPtag as an Orally Bioavailable, Hyperstable Epitope Tag and Generalizable Protein Binding Scaffold. Biochemistry. 2018 May 29;57(21):3036-3049.