Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000552)
SBP Name
Knottin anti-Integrin 7E
Synonyms
Knottin 7E
Molecular Weight 4.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Pichia pastoris
Selection Method Yeast display
Highest Status Research
Sequence Length 38
SBP Sequence
>Knottin anti-Integrin 7E
GCVRLHESCLGQQVPCCDPAATCYCVGRGDDNLKCYCR
Sequence Description Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VIII form disulfide bonds; Cys VI and Cys VII form disulfide bonds.
3D Structure
Computationally Modelled Structure
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Template Name AgRP
Template Sequence Description Cys I and Cys IV form disulfide bonds; Cys II and Cys V form disulfide bonds; Cys III and Cys VIII form disulfide bonds; Cys VI and Cys VII form disulfide bonds.
Protein Scaffold Information of This SBP
Scaffold ID PS042
Scaffold Info
[1]
Scaffold Name Knottin
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Integrin alpha-v-beta-3
BTS Info
Binder Solid tumour/cancer [ICD-11: 2A00-2F9Z] Kd: 1.6 nM Stanford University [1]
References
1 Engineered cystine-knot peptides that bind alpha(v)beta(3) integrin with antibody-like affinities. J Mol Biol. 2009 Jan 30;385(4):1064-75.