General Information of Synthetic Binding Protein (SBP) (ID: SBP000309)
SBP Name
SWEEPin anti-ySUMO clone 31
Synonyms
SWEEPin SUMO-31
Molecular Weight 11.6 kDa
Thermal Denaturation TEMP 59.9 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 99
SBP Sequence
>SWEEPin anti-ySUMO clone 31
GSGEWEIIDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPSMKKTIYDDYYVDRIKGY
EYQLYVYASDKLFRADISEDYWSNGYRKLLRFNGPVPPP
3D Structure
Computationally Modelled Structure
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Template Name scMonellin
Protein Scaffold Information of This SBP
Scaffold ID PS059
Scaffold Info
[1]
Scaffold Name SWEEPin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Yeast small ubiquitin-related modifier
BTS Info
Binder Research tool Kd: 3500 nM Okayama University [1]
References
1 A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins. J Biochem. 2021 Jan 2;mvaa147.