Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000019)
SBP Name
Monobody anti-ySUMO clone 9
Synonyms
ySMB-9
Molecular Weight 10.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 3RZW
Sequence Length 95
SBP Sequence
>Monobody anti-ySUMO clone 9
GSGSSVSSVPTKLEVVAATPTSLLISWDAGYWFIDYYRITYGETGGNSPVQEFTVPGYSS
TATISGLSPGVDYTITVYAYYDNYGWSPISINYRT
3D Structure
Experimentally Validated Structure
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Template Name Fibronectin type III domain (FN3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Yeast small ubiquitin-related modifier
BTS Info
Inhibitor Research tool Kd: 8000 nM University of Chicago [1]
References
1 Isoform-specific monobody inhibitors of small ubiquitin-related modifiers engineered using structure-guided library design. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7751-6.